ABSTRACT
Objective@#To investigate the prevalence and influencing factors of lower respiratory tract disease.@*Methods@#The health physical examination data of 4000 coal dust exposed workers who had physical examination in the Institute of Occupational Disease Prevention and Treatment in 2016 were collected and analyzed with SPSS 20.0 software.@*Results@#Out of the 4000 coal workers, the Prevalence rate of pulmonary tuberculosis, chronic bronchitis, CWP were 1.00% (40/4000) , 0.63% (25/4000) , 0.43% (17/4000) . 17 coal workers suffered from coal workers. The prevalence of workers both exposed to silicon and coal dust was 1.15% (2/174) , which was higher than that of other the job type. The prevalence of CWP among age groups, length of service and occupational category were found significant difference (P<0.05) , raising with the increase of duration of dust exposure and age increasing (χ2trend=17.171, 5.344, P<0.05) . The prevalence of emphysema and chronic bronchitis between different working years and different ages are all statistically significant (P<0.05) , increasing with duration of dust exposure (χ2trend=9.906, 10.118, P<0.05) .@*Conclusion@#The prevalence rate of CWP is not high, and closely related with duration of dust exposure and dust species. The prevalence of lower respiratory tract disease is higher in the tunneling and mining workers and raising with the duration of dust exposure.
ABSTRACT
Objective@#To explore the association between dust exposure and the incidence of hypertension in male coal miners.@*Methods@#Using the method of retrospective cohort study,a hypertension cohort of colliery in Henan Province was established in January 2006. From 2006 to 2017,all the male coal miners in a colliery who were exposed to dust were selected into the exposure group including tunneling, mining,auxiliary and combining workers, and workers from administrative logistics departments who were not exposed to dust were selected into the control group. The eligible participants should satisfy following conditions: working more than one year, with clear and complete record of occupation change, and with complete archives and reliable diagnosis of occupational health surveillance. The exclusion criteria of participants were with hypertension at the baseline of study or with heart,liver,kidney diseases and malignant tumors. A total of 12 647 participants were enrolled in this study (11 663 in the exposure group and 984 in the control group). The follow-up period was from January 2006 to December 2017,with a total follow-up of 89 259.75 person-years. Questionnaires and physical measurements were used to collect general demographic characteristics, occupational exposure history and occupational health surveillance data of all participants. The Cox proportional hazards regression model was used to estimate the association between the dust exposure and the incidence of hypertension.@*Results@#During the follow-up period, 2 549 new-onset hypertension patients were identified with an incidence density of hypertension about 2 855.71 per 100 000 person-years. The incidence density of hypertension was 2 967.58 per 100 000 person-years in the exposure group, and 1 643.85 per 100 000 person-years in the control group. The results of multivariate Cox proportional hazards regression model showed that after the adjustment of marriage, age, smoking, alcohol drinking and body mass index,the risk of hypertension was higher in the exposure group compared with the control group (HR=1.692, 95%CI: 1.410-2.032). Further analysis showed that compared with workers from administrative logistics departments,the risk of hypertension in tunneling,mining and auxiliary working was 1.629(1.345-1.973),1.677(1.374-2.046) and 1.782(1.475-2.151),respectively.@*Conclusion@#Dust exposure may increase the risk of hypertension in male coal miners.
ABSTRACT
Objective To investigate the value of combined detection of serum cystatin C(Cys-C),alpha 1- microspheres(α1-MG)and urinary microalbumin(mAlb)in the early stage of renal damage in patients with primary hypertension(PH).Methods One hundred and twenty-eight patients with PH were divided into 3 groups according to urinary albumin excretion rate(UAER),group I,normal proteinuria,47 cases,group II microalbuminuria,43 cases,group III,massive proteinuria,38 cases and 50 healthy subjects(the control group)served as research object.Hitachi 7180 automatic biochemical analyzer was applied with latex turbidimetric immunoassay to detect serum Cys-C,α1-MG and mAlb,serum creatinine(Crea)and serum urea (Urea)were determined by enzyme method.Comparative analysis on the early diagnosis efficiency of renal damage was made o based on the combined detection results.Results PH patients were tested by Cys-C( (0.98±0.13)mg/L vs.(1.62±0.55)mg/L vs.(3.17±1.04)mg/L),α1-MG((29.32±6.46)mg/L vs. (38.58±11.79)mg/L vs.(61.42±22.71)mg/L),mAlb((26.35±6.53)mg/24 h vs.(54.24±12.78)mg/24 h vs.(373.6±98.40)mg/24 h),Crea((5.06±0.67)μmol/L vs.(89.64±28.84)μmol/L vs.(164.16 ±58.77)μmol/L),Urea((5.06 ± 0.67)mmol/L vs.(7.87 ± 2.95)mmol/L vs.(15.10 ± 7.41)mmol/L, compared with those in the control group((0.69±0.08)mg/L,(17.15±4.30)mg/L,(19.55±4.28)mg/24 h,(68.39±20.11)μmol/L,(5.05±0.78)mmol/L,there were significant differences among the four groups(F=164.74,93.01,553.27,61.38,65.24,P=0).The results of Cys-C,α1-MG and mAlb in patients with PH were higher than those in the control group(P<0.05,P<0.01).The results of Cys-C,α1-MG and mAlb in group II were higher than those in group I,but lower than those in group III(P<0.01).The detection results of Crea in group I and group II were higher than those in the control group(P>0.05),but lower than that in group III(P<0.05).The result of Urea in group I was higher than that in the control group(P>0.05),but lower than those in group II and group III(P<0.05).Crea Cys-C and Urea were positively correlated(r=0.385,r=0.310,P=0.006,P=0.025),α1-MG was positively correlated with Crea,Urea(r=0.310,r=0.228,P=0.028,P=0.043); mAlb was positively correlated with Crea and Urea(r=0.382,r=0.302,P=0.006,P=0.033). The specificity of combined detection of three markers(55.3%)was lower than the individual detection(74.5%,68.1%,80.9%)and also lower than the two-unit(70.2%,63.8%,index 78.7%); its sensitivity(90.7%)was significantly higher than that of the individual detection(65.1%,58.1%,67.4%)and the two-unit(79.1%,74.4%,69.8%).Conclusion Cys-C,α1-MG and mAlb can be used as important indicators in the diagnosis in the patients with PH renal damage,which can increase the effectiveness PH early renal damage diagnosis,can be used for early detection of renal damage in PH patients,with the disease monitoring.
ABSTRACT
Objective To establish a method for quality control of oleanolic acid and ursolic acid in Qingdu tablet. Methods The content of oleanolie acid and ursolic acid in Qingdu tablet were determined by high performance liquid chroma-tography(HPLC). The analytical column was kromasil-C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-0.5% phosphoric acid solution (88:12). The UV detection wavelenghth was 210 nm. The flow rate was 1.0 ml/min. Results Oleanolic acid and ursolic acid showed a good linearity in range of 0.06-0.54μg (R=0.999 3) and 0.30-2.70μg (R=0.999 7). The average recovery rates of Oleanolic acid and ursolic acid were 100.48% and 102.21%, respectively. Conclusions This method is simple, rapid and accurate. It could be used for the quality control and quality study of the Qingdu tablet.
ABSTRACT
Objective To investigate miR-146a-Smad4 expression during ultraviolet A(UVA)-induced photoaging of human skin fibroblasts (HSFs), and to evaluate effects of up-regulation of miR-146a expression on its target gene Smad4 and cell photoaging. Methods HSFs were isolated from the prepuce, and subjected to primary culture and maintained up to 10th passage. Then, the HSFs were classified into 4 groups: blank control group receiving no treatment, UVA group irradiated with 10 J/cm2 UVA, miR-146a group transfected with a lentiviral vector expressing miR-146a, UVA+ miR-146a group transfected with the lentiviral vector expressing miR-146a followed by UVA radiation. Real time PCR was performed to measure miR-146a expression in HSFs in the UVA group on day 0, 3, 7 and 14 after UVA radiation.Fluorescence microscopy was carried out to estimate transfection efficiency on day 7 and 14 in the miR-146a group after transfection, and real time PCR was performed to quantify miR-146a expression in these cells. Methyl thiazolyl tetrazolium (MTT)assay was conducted to evaluate proliferative activity of HSFs, real time PCR to quantify mRNA expressions of photoaging-related genes p53, p21 and p16, and Western blot analysis to measure Smad4 protein expression in these cells. Statistical analysis was carried out by using repeated-measures analysis of variance and factorial design analysis of variance. Results Repeated-measures analysis of variance showed that the expression of miR-146a decreased over time in both the UVA group and blank control group(F = 213.840, P 0.05). Factorial design analysis of variance showed that UVA radiation had an inhibitory effect on the proliferative activity of HSFs (P 0.05). Real time PCR and Western blot analysis both revealed that UVA radiation could increase the expressions of p53, p21 and p16 mRNAs as well as Smad4 protein(all P 0.05). Conclusion The expression of miR-146a is inhibited in UVA-induced photoaged HSFs, and its up-regulation may counteract cell photoaging by suppressing Smad4 expression in, and promoting proliferation of, photoaged HSFs.
ABSTRACT
Objective To investigate the size and diversification of peripheral nerve measured by high frequency ultrasound in di-abetic peripheral neuropathy patient .Methods The endings of the medial branch of deep peripheral nerves(DPN) were interrogated by high frequency ultrasound and nerve electrophysiology ,and the nerve conduction characters were studied in a cohort of 150 clini-cally diagnosed diabetic peripheral neuropathy patients and a control group of 100 healthy volunteers .At the sametime these cases underwent electrophysiological examination .Results Distinct echoic appearances were consistently detected between the DPN nerves of diabetic peripheral neuropathy patients and healthy volunteers .The sensitivity rate of high frequency ultrasound and nerve electrophysiological examination in diagnosis of diabetic peripheral neuropathy were 91 .33% and 74 .67% ,and the specificity rate were 91% and 82% .Conclusion High frequency ultrasound may play a role in the diagnosis of diabetic peripheral neuropathy .
ABSTRACT
0.05).Conclusion:Ionizing radiation-induced color change combined with chrominance technique may provide a new convenient method for studying radiation absorbed dose.